Membrane integration of E. coli model membrane proteins

Computational Studies on Lactose Permease of E. Coli as a Model for Membrane Transport Proteins

Title of Document: COMPUTATIONAL STUDIES ON LACTOSE PERMEASE OF E. COLI AS A MODEL FOR MEMBRANE TRANSPORT PROTEINS Pushkar Yashvant Pendse, Doctor of Philosophy, 2013 Directed By: Jeffery B Klauda, Assistant Professor, Department of Chemical and Biomolecular Engineering Membrane transport proteins actively transport ions, metabolites, drug molecules, and others across the amphiphilic cell membr...

Biogenesis of Polytopic Membrane Proteins: Membrane Segments Assemble within Translocation Channels prior to Membrane Integration

The initial steps in the biogenesis of membrane proteins parallel that of secretory proteins. The translocation of membrane proteins, however, must be interrupted prior to the complete traversal of the membrane. This is followed by their folding and integrating into the lipid bilayer. We have previously shown that as each latent transmembrane segment (TMS) in a polytopic membrane protein emerge...

Cloning and expression of Brucella outer membrane protein 36kDa (OMP2b) in E. coli

Background & Objective: Brucellosis is an important zoonotic disease of economic significance. Brucella species are gram-negative, facultative intracellular bacteria, and are capable of replicating in the phagosomes of macrophages. They cause infection in several animal species and humans. Prevention of new diseases and diagnosis of cases infected with the organism are both essential for eradic...

Use of Membrane Technology in Bioprocessing Therapeutic Proteins from Inclusion Bodies of E. coli

MARCH 2009 The ultimate goal of recombinant fermentation research is costeffective production of desired proteins by maximizing volumetric productivity (to obtain the highest amount of protein in a given volume in the least amount of time). Bioprocessing for recombinant proteins using genetically modified organisms requires a stable, high-yielding recombinant culture, a highly productive fermen...

A Chimeric Vaccine Consisting of Highly Immunogenic Regions form Escherichia coli Iron Regulated Outer-Membrane Proteins: An In Silico Approach